How To Make A MEGA-plate

Overview

Replace this sentence with a brief description of the protocol and its goal.

The goal is hoe to build a MEGA-plate.

Materials

For a plate of 60cm on 80cm:

• Polycarbonate sheet 60cm X 80cm
• Plexiglas sheet 30cm X 60cm
• Dichloromethane

• Syringe
• Needle
• 9 X 2L Erlenmeyer flask

Procedure

1. Plan the design of the plate.
   1. 
2. Perform thermocycling program
   1. 95 °C 5 min
   2. 95 °C 30 s
   3. T_H 30 s
   4. 72 °C 1 min for each 1 kb PCR product
   5. Repeat steps 2-4 a total of 12-36 times (24 is standard).
   6. 72 °C 5 min
   7. 12 °C hold

Notes

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References

Relevant papers and books

1. Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
2. Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
3. Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164

Contact

• Who has experience with this protocol?

or instead, discuss this protocol.