How to read the memory state?: Difference between revisions
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However, there's one problem we'll have to face: if relying on transcriptionnal activation, the time of synthesis plus the time of maturation of the FP (around 20 min at best) would preclude very rapid reading. | However, there's one problem we'll have to face: if relying on transcriptionnal activation, the time of synthesis plus the time of maturation of the FP (around 20 min at best) would preclude very rapid reading. | ||
(maybe not true see Hasty paper on fast oscillator) | |||
=alternative possibilities:= | =alternative possibilities:= | ||
Revision as of 19:06, 20 November 2008
Back to Jerome's notebook
Introduction
Reading the memory state would probably relies on fluorescent proteins (FPs).(it would however be nice to keep a hard record of all event in DNA structure for example). Advantage that it would keep the cells alive, quite easy to read and numerous variants are available. By using Spectral deconvolution, weare virtually able to separate all overlapping spectras, so that we can have numerous different colors.
note
However, there's one problem we'll have to face: if relying on transcriptionnal activation, the time of synthesis plus the time of maturation of the FP (around 20 min at best) would preclude very rapid reading. (maybe not true see Hasty paper on fast oscillator)
alternative possibilities:
- Change subcellular localisation: Cell/nucleus, cytosol/membrane (more applicable to trans-kingdom), inta/extra cellular (!!!).
- Activable fluorophore: the protein is here, but its fluorescence quenched. Writing state change protein conformation or sequester/destroy the quencher.
see if Dark quencher could work.article
