Huang: DH5alpha transformation

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(New page: 1. Set tubes of frozen cells on ice and let thaw very slowly 2. Add 100ul thawed cells to new eppendorf 3. Add 1-2ul DNA to cells 4. Incubate on ice for 20 min 5. Heat shock in 42C water b...)
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Revision as of 19:47, 1 April 2009

1. Set tubes of frozen cells on ice and let thaw very slowly 2. Add 100ul thawed cells to new eppendorf 3. Add 1-2ul DNA to cells 4. Incubate on ice for 20 min 5. Heat shock in 42C water bath for one min (make sure water bath is at exactly 42C) 6. Incubate on ice 2 min 7. Add 900ul growth medium (LB) and shake 1 hour at 37C 8. Plate about 200ul and grow overnight

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