HughesLab:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (13:00, 5 July 2017) (view source)
(Fly Behavior and Genetics)
 
(10 intermediate revisions not shown.)
Line 3: Line 3:
== Molecular Biology ==
== Molecular Biology ==
-
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
+
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:RNA_prep_using_Trizol.docx]]<br>
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
Line 12: Line 12:
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
<br>
<br>
-
<br>
+
 
== Fly Behavior and Genetics ==
== Fly Behavior and Genetics ==
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
-
* Vials for DAM analysis:  
+
* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]]
 +
* Fly food protocol: [[Image:Fly_food.docx]]
 +
<br>
 +
== Analytics ==
 +
* Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]]
 +
* RUM (RNA-Seq Unified Mapper) guide: [[Image:RUM guide.pdf]]
 +
* Basic linux commands: [[Image:linux commands.pdf]]
<br>
<br>
<br>
<br>
-
 
-
== Analytics ==
 
-
* Human temperature record analysis:
 
-
 
-
 
----
----
-
[[Image:UMSL_logo.jpg|left|100px]]
+
[[Image:WashU_Logo.png|left|150px]]
-
<p style="font-size: 150%;">
+
<p style="font-size: 170%;">
Hughes Lab<br>
Hughes Lab<br>
-
[http://www.umsl.edu/~biology/ Department of Biology]<br>
+
[https://pulmonary.wustl.edu/ Pulmonary and Critical Care Medicine]<br>
-
University of Missouri, St. Louis<br>
+
Washington University School of Medicine
-
(Starting in August, 2013)
+

Current revision

Home        Lab Members        Research        JTK_Cycle        CircaDB        MuscleDB        Publications        Protocols        Courses        Academic Analytics        Open Positions        Contact       



Contents

Molecular Biology


Next-generation sequencing


Fly Behavior and Genetics


Analytics




Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine

Personal tools