HughesLab:Protocols

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== Fly Behavior and Genetics ==
 
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== Molecular Biology ==
== Molecular Biology ==
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes:  [[Image:QPCR_protocol.docx]]<br>
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* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp.docx]]
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* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br>
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* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br>
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* RNA prep using Trizol and Qiagen RNeasy columns: [[Image:RNA_prep_using_Trizol.docx]]
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== Next-generation sequencing ==
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* Pilot protocol for PCR amplifying fly RNA editing sites:  [[Image:editingseq_pilot.docx]]<br>
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== Fly Behavior and Genetics ==
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* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]]
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* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]]
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== Next-generation sequencing ==
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== Analytics ==
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* Pilot protocol for PCR amplifying fly RNA editing sites: [[Image:editingseq_pilot.docx]]<br>
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* Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]]
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Hughes Lab<br>
[http://www.umsl.edu/~biology/ Department of Biology]<br>
[http://www.umsl.edu/~biology/ Department of Biology]<br>
University of Missouri, St. Louis<br>
University of Missouri, St. Louis<br>
(Starting in August, 2013)
(Starting in August, 2013)

Revision as of 11:42, 13 February 2013

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Contents

Molecular Biology


Next-generation sequencing


Fly Behavior and Genetics


Analytics




Hughes Lab
Department of Biology
University of Missouri, St. Louis
(Starting in August, 2013)

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