HughesLab:Protocols

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Molecular Biology

• Basic qPCR protocol using the ABI7900HT machine and Taqman probes: File:RNA prep using Trizol.docx
  
• Arcturus RiboAmp RNA amplification from fly brains: File:RiboAmp1.docx
  
• Basic RT reaction using ABI Archive cDNA kit: File:RT protocol.docx
  
• RNA prep using Trizol and Qiagen RNeasy columns: File:RNA prep using Trizol.docx

Next-generation sequencing

• Pilot protocol for PCR amplifying fly RNA editing sites: File:Editingseq pilot.docx
  

Fly Behavior and Genetics

• Drosophila virgin collection: File:Drosophila virgin collection.docx
• Vials for DAM analysis: File:Fly food for DAM analysis.docx

Analytics

• Human temperature record analysis: File:Human Temp Analysis.docx
• RUM (RNA-Seq Unified Mapper) guide: File:RUM guide.pdf
• Basic linux commands: File:Linux commands.pdf

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Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine