Hygromycin B: Difference between revisions
(resistance) |
Ewa Szczesna (talk | contribs) No edit summary |
||
| (6 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells. | '''Hygromycin B''' is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells. | ||
== Mode of action == | == Mode of action == | ||
| Line 8: | Line 8: | ||
== Stock solution == | == Stock solution == | ||
*Stock Solution Concentration: 50 mg/mL | |||
*Solvent: H2O | |||
== Stability == | == Stability == | ||
* >1y at –20°C | * >1y at –20°C | ||
* >2y at +4°C (http://www.hygromycin.com/service.htm) | |||
* 3m at RT | * 3m at RT | ||
* >1m at 37°C | * >1m at 37°C | ||
| Line 16: | Line 19: | ||
== Usage notes == | == Usage notes == | ||
The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 | The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 μg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below. | ||
{| | {| border=1 cellpadding=5 cellspacing=0 | ||
|+ '''typical HygB concentrations by cell type''' | |||
|- | |- | ||
! cell type | ! cell type | ||
! | ! HygB concentration | ||
|- | |- | ||
| mammalian cells | | mammalian cells | ||
| 200 | | 200 μg/ml | ||
|- | |- | ||
| plant cells & bacteria | | plant cells & bacteria | ||
| 20-200 | | 20-200 μg/ml | ||
|- | |- | ||
| fungi | | fungi | ||
| 200-1000 | | 200-1000 μg/ml | ||
|} | |} | ||
Latest revision as of 02:48, 23 July 2012
Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells.
Mode of action
Interferes with protein synthesis.
Mechanism of resistance
Resistance genes are kinases that inactivate Hygromycin B through phosphorylation.
Stock solution
- Stock Solution Concentration: 50 mg/mL
- Solvent: H2O
Stability
- >1y at –20°C
- >2y at +4°C (http://www.hygromycin.com/service.htm)
- 3m at RT
- >1m at 37°C
sensitive to high concentrations of acid, but short-term exposure to dilute acids tolerated;
Usage notes
The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 μg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.
| cell type | HygB concentration |
|---|---|
| mammalian cells | 200 μg/ml |
| plant cells & bacteria | 20-200 μg/ml |
| fungi | 200-1000 μg/ml |
For more information on working concentrations: [1]
External links
Wikipedia entry: Hygromycin B
Information from hygromycin.net (Invivogen)
