Hygromycin B

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(table layout)
(stability at +4C added with reference)
Line 11: Line 11:
== Stability ==
== Stability ==
* >1y at –20°C
* >1y at –20°C
 +
* >2y at +4°C (http://www.hygromycin.com/service.htm)
* 3m at RT
* 3m at RT
* >1m at 37°C
* >1m at 37°C

Revision as of 06:28, 21 August 2009

Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells.

Contents

Mode of action

Interferes with protein synthesis.

Mechanism of resistance

Resistance genes are kinases that inactivate Hygromycin B through phosphorylation.

Stock solution

Stability

sensitive to high concentrations of acid, but short-term exposure to dilute acids tolerated;

Usage notes

The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 mg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.

typical HygB concentrations by cell type
cell type HygB concentration
mammalian cells 200 mg/ml
plant cells & bacteria 20-200 mg/ml
fungi 200-1000 mg/ml

For more information on working concentrations: [1]

External links

Wikipedia entry: Hygromycin B

Information from hygromycin.net (Invivogen)

  • mechanism of action: [2]
  • resistance genes: [3]
  • chemistry, stability: [4]

References

Personal tools