IC Bioinfs/Project1/Registry

Background on Biobricks

Definition

• See links in 1 Main Page under "Resources"

Types of Biological Parts

• How to create a basic biobrick: http://partsregistry.org/Basic_Biobrick%2C_example_1

Promoter

• Promoter Catalog
  • Classified by function:
    • Constitutive promoters: These promoters are active independent of transcription factors, and are "on" by default.
    • Cell signalling: The registry has a set of promoters related to sending and receiving signals between different cells.
    • Metal sensitive: This set includes promoters that are sensitive to various metals. The promoters are typically regulated by a receptor protein that binds to the metal ion or complex.
    • Phage promoters: A collection of all phage promoters available from the registry. The promoters are often used for very high expression of a protein. These promoters work in E. coli and other chassis but typically require a particular RNA polymerase to be present.
    • IIT Madras Stresskit promoters: a well-characterized collection of negatively regulated E. coli promoters that have been engineered to be recognized by alternative σ factors. This collection was developed by the 2008 IIT Madras iGEM team.
  • Classified by regulation and RNA polymerase:

RBS

See relevant catalogue part

Coding

See relevant catalogue part

Composites

These parts are made up of basic parts.

Understanding Entries and Basic information

Short Form: Promoters

Sample Entry:Promoter

Sequence Analysis

• This tool is used to compare several sequencing reaction results to the expected sequece of a part
• In the lab, they perform alignments to confirm that the sequence they have is correct.

Hard Facts

• Repeat of Main page- See example http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa_I13453
• Part parameters- Positive/Negatively regulated, hill coefficient, pops, promoter degradation rate...