IGEM:American University/2009/Notebook/AU Quantum Dot Lab/2014/09/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/09/08 Entry for IGEM:American_University/2009/Notebook/AU_Quantum_Dot_Lab)
 
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
==Entry title==
==Objective==
* Insert your content here.
* Synthesize HgS NCs
* Analyze the UV-vis and Fluorescence Spectra
 
==Solution Preparation==
====5 mM Mercurous Nitrate====
*Mass needed: 0.0701g
*Mass measured: 0.0705g
*Volume: 50mL volumetric
*Final Molarity: 0.00502 M HgNO3
<br>
After vigorous stirring the HgNO3 would not dissolve in, and therefore HgCl2 would be substituted for this trial.
 
====5mM Mercuric Chloride====
*Mass needed: 0.0679g
*Mass measured:0.0670g
*Volume: 50mL volumetric
*Final Molarity: 0.00494 M HgCl2
 
====(15mg/mL) BSA====
*Mass needed: 0.75g
*Mass measured: 0.751g
*Volume: 50mL volumetric
Final Molarity: 15mg/mL
 
====1M Sodium Hydroxide====
*Mass needed: 2g
*Mass Measured:1.98g
*Volume: 50mL volumetric
*Final molarity 0.99 M NaOH
 
==Protocol==
# In a round bottom flask 5mL of 5mM HgNO3
# 5mL BSA solution in MQ water and stir
# Correct the pH to approximately 9 using 1M NaOH
# Allow solution to stir overnight (12-18 hours)
# Add 4mL of 20mM Na2S and stir for 15 minutes
 
==Notes==
* The mercuric chloride with a 4+ charge vs Mercurous nitrate with a 2+ charge, could explain the poor results seen in both fluorescence and UV-vis spectra.  




Revision as of 10:51, 15 September 2014

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Synthesize HgS NCs
  • Analyze the UV-vis and Fluorescence Spectra

Solution Preparation

5 mM Mercurous Nitrate

  • Mass needed: 0.0701g
  • Mass measured: 0.0705g
  • Volume: 50mL volumetric
  • Final Molarity: 0.00502 M HgNO3


After vigorous stirring the HgNO3 would not dissolve in, and therefore HgCl2 would be substituted for this trial.

5mM Mercuric Chloride

  • Mass needed: 0.0679g
  • Mass measured:0.0670g
  • Volume: 50mL volumetric
  • Final Molarity: 0.00494 M HgCl2

(15mg/mL) BSA

  • Mass needed: 0.75g
  • Mass measured: 0.751g
  • Volume: 50mL volumetric

Final Molarity: 15mg/mL

1M Sodium Hydroxide

  • Mass needed: 2g
  • Mass Measured:1.98g
  • Volume: 50mL volumetric
  • Final molarity 0.99 M NaOH

Protocol

  1. In a round bottom flask 5mL of 5mM HgNO3
  2. 5mL BSA solution in MQ water and stir
  3. Correct the pH to approximately 9 using 1M NaOH
  4. Allow solution to stir overnight (12-18 hours)
  5. Add 4mL of 20mM Na2S and stir for 15 minutes

Notes

  • The mercuric chloride with a 4+ charge vs Mercurous nitrate with a 2+ charge, could explain the poor results seen in both fluorescence and UV-vis spectra.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:American_University/2009/Notebook/AU_Quantum_Dot_Lab/2014/09/08&oldid=820419"