IGEM:American University/2009/Notebook/AU Quantum Dot Lab/2014/09/15: Difference between revisions
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==Protocol== | ==Protocol== | ||
# In a round bottom flask 5mL of 5mM HgNO3 | |||
# 5mL BSA solution in MQ water and stir | |||
# Correct the pH to approximately 9 using 1M NaOH | |||
# Allow solution to stir overnight (12-18 hours) | |||
# Add 4mL of 20mM Na2S and stir for 15 minutes | |||
==Notes== | |||
*Solutions for this synthesis were prepared by James C. Schwabacher | |||
*The HgNO3 was still difficult to fully dissolve in solution and therefore it was sonicated until almost homogenous to be used | |||
*The color of the bio-conjugates was light grey and will be monitored as it should be pale yellow after stirring. | |||
Revision as of 11:06, 15 September 2014
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Objective
Protocol
Notes
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