IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/02/01 - Revision history

Noah Benjamin: /* Today's Procedure */

Noah Benjamin — Wed, 06 Feb 2013 18:11:32 GMT

Today's Procedure

←Older revision		Revision as of 18:11, 6 February 2013
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	=Today's Procedure=		=Today's Procedure=

-	7 films were made using 0.5g PVOH (MW: 146K-186K) dissolve in 6+ mL water (more water was added as it became apparent more water was needed). ~~Usual glutaraldehyde procedures~~ were ~~used~~.	+	7 films were made using 0.5g PVOH (MW: 146K-186K) dissolve in 6+ mL water (more water was added as it became apparent more water was needed). The amounts of isopropanol added were determined based on a calculation relating number of OH groups in PVOH to OH groups in Isopropanol. The OH groups in PVOH will react with the OH groups in isopropanol in a dehydration reaction. Calculations below.

	1) PVOH and water		1) PVOH and water
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	2) PVOH, 1mL glutaraldehyde		2) PVOH, 1mL glutaraldehyde

-	3) PVOH, 1mL glutaraldehyde, 0.069g Isopropanol	+	3) PVOH, 1mL glutaraldehyde, 0.069g Isopropanol (1 isopropanol OH group: 10 PVOH OH group)

-	4) PVOH, 1mL glutaraldehyde, 0.0069g isopropanol	+	4) PVOH, 1mL glutaraldehyde, 0.0069g isopropanol (1 isopropanol OH group: 100 PVOH OH group)

-	5) PVOH, 1mL glutaraldehyde, 0.00069g isopropanol	+	5) PVOH, 1mL glutaraldehyde, 0.00069g isopropanol (1 isopropanol OH group: 1,000 PVOH OH group)

-	6) PVOH, 1mL glutaraldehyde, 0.000069g isopropanol	+	6) PVOH, 1mL glutaraldehyde, 0.000069g isopropanol (1 isopropanol OH group: 10,000 PVOH OH group)

	7) PVOH and water in a small dish		7) PVOH and water in a small dish

-	Isopropanol was measured using a serial dilution of pure isopropanol, 10% isopropanol, 1%isopropanol, and 0.1% isopropanol.	+	Isopropanol was measured using a serial dilution of pure isopropanol, 10% isopropanol, 1%isopropanol, and 0.1% isopropanol. First the PVOH was dissolved in water, then the isopropanol was added/mixed in thoroughly, finally the glutaraldehyde was added under the hood and stirred in thoroughly.
		+

	This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.		This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.

-	~~<b>[[User:Matt Hartings\|Matt Hartings]]What are the "usual glutaraldehyde procedures~~. ~~At least put a link~~. ~~Also~~, ~~why did you choose those amounts~~ of isopropanol~~? How did you make those calculations? What do those numbers mean?<~~/b>	+	=Calculations=
		+
		+	(166000g/mole PVOH)/(44g/mole per repeating OH unit in PVOH)=3772.74 OH groups in 1 mole of PVOH.
		+	(0.5g PVOH)/(166000g/mole)(3773 OH groups)(6.022E23 molecules/mole)=6.865E21 OH groups in 0.5g of PVOH.
		+	To solve for amount of isopropanol added to solutions, 6.865E21 OH groups in 0.5g PVOH was multiplied by the ratio of OH isopropanol groups per OH PVOH groups desired. This number was then divided by 6.022E23 molecules/mole to yield moles of isopropanol desired. This is then multiplied by the molecular weight of isopropanol (60.1g/mole) to yield the desired mass of isopropanol added.
		+
		+

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Matt Hartings: /* Today's Procedure */

Matt Hartings — Wed, 06 Feb 2013 00:58:13 GMT

Today's Procedure

←Older revision		Revision as of 00:58, 6 February 2013
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	This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.		This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.

-		+	<b>[[User:Matt Hartings\|Matt Hartings]]What are the "usual glutaraldehyde procedures. At least put a link. Also, why did you choose those amounts of isopropanol? How did you make those calculations? What do those numbers mean?</b>

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Noah Benjamin: /* Welcome to Advanced Experimental Chemistry */

Noah Benjamin — Fri, 01 Feb 2013 19:41:52 GMT

Welcome to Advanced Experimental Chemistry

←Older revision		Revision as of 19:41, 1 February 2013
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	\| colspan="2"\|		\| colspan="2"\|
	==Welcome to Advanced Experimental Chemistry==		==Welcome to Advanced Experimental Chemistry==
-	to ~~be further editted~~	+
		+
		+	=Today's Procedure=
		+
		+	7 films were made using 0.5g PVOH (MW: 146K-186K) dissolve in 6+ mL water (more water was added as it became apparent more water was needed). Usual glutaraldehyde procedures were used.
		+
		+	1) PVOH and water
		+
		+	2) PVOH, 1mL glutaraldehyde
		+
		+	3) PVOH, 1mL glutaraldehyde, 0.069g Isopropanol
		+
		+	4) PVOH, 1mL glutaraldehyde, 0.0069g isopropanol
		+
		+	5) PVOH, 1mL glutaraldehyde, 0.00069g isopropanol
		+
		+	6) PVOH, 1mL glutaraldehyde, 0.000069g isopropanol
		+
		+	7) PVOH and water in a small dish
		+
		+	Isopropanol was measured using a serial dilution of pure isopropanol, 10% isopropanol, 1%isopropanol, and 0.1% isopropanol.
		+
		+	This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.

Noah Benjamin: Autocreate 2013/02/01 Entry for IGEM:American_University/2009/Notebook/Advanced_Experimental_Chemistry:_Fun_Times_in_Beeghly

Noah Benjamin — Fri, 01 Feb 2013 19:31:08 GMT

Autocreate 2013/02/01 Entry for IGEM:American_University/2009/Notebook/Advanced_Experimental_Chemistry:_Fun_Times_in_Beeghly

New page

{|{{table}} width="800"
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==Welcome to Advanced Experimental Chemistry==
to be further editted


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