IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/04/23

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(Welcome to Advanced Experimental Chemistry)
Current revision (11:44, 23 April 2013) (view source)
(Welcome to Advanced Experimental Chemistry)
 
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1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible.
1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible.
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2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer. Set in the electrophoresis tray with the comb for 20+ minutes.
+
2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer (microwaved together for 40 seconds). Set in the electrophoresis tray with the comb for 20+ minutes.

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Welcome to Advanced Experimental Chemistry

1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible.

2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer (microwaved together for 40 seconds). Set in the electrophoresis tray with the comb for 20+ minutes.



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