IGEM:American University/2009/Notebook/BCHM 2/2017/02/22: Difference between revisions
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== | ==BSA Templated CdS Quantum Dot synthesis and Myoglobin templated ZnS quantum dots data== | ||
CdS quantum dots were synthesized in a BSA template and fluorescence scans were run on the previously synthesized myoglobin templated ZnS quantum dots. | |||
==Procedure== | |||
#A Cadmium acetate dihydrate solution was made to replace the previous stock solution of cadmium acetate anyhydrous because the cadmium acetate came out of solution and was not uniform. | |||
##0.666g of cadmium acetate dihydrous was dissolved in a 500mL volumetric flask. The stock solution is 5mM. | |||
#Sample was made BSA: 0.1mM cadmium acetate, 3.125uM BSA, 10mM potassium thioacetate 5mL total | |||
##0.5M potassium thioacetate | |||
###(10mM)(5.0mL)=(XmL)(0.5mM) | |||
###XmL= 100uL potassium thioacetate | |||
##BSA 63.1uM | |||
###(3.125uM)(5.0mL)=(XmL)(63.1uM) | |||
###X=0.247mL= 247uL BSA | |||
##Cadmium Acetate 0.5M | |||
###(0.1mM)(5.0mL)=(XmL)(50mM) | |||
###X= 500uL | |||
##Water | |||
###Volume of water= 5000uL- 100uL-247uL-500uL= 4153uL | |||
==Myoglobin templated ZnS Quantum dots== | |||
Scans taken of myoglobin templated ZnS quantum dots pH 4-10 | |||
[[Image:ALL together Anneliese faustino 22217.PNG]] | |||
[[Image:ALL together anneliese 22217.PNG]] | |||
==Notes== | |||
Since the myoglobin templated CdS quantum dots did not appear to have fully formed after stirring for one day, scans were only taken after stirring for 7 days. | |||
==Overview== | |||
[[Image:ALL together Anneliese faustino 22217.PNG]] | |||
[[Image:ALL together anneliese 22217.PNG]] | |||
==Data Analysis: In Depth== | |||
pH 4 | |||
[[Image:PH 4 22217anneliese.PNG]] | |||
pH 4 Selected View | |||
[[Image:PH 4 view anneliese anneliese anneliese.PNG]] | |||
pH 5 | |||
[[http://www.openwetware.org/wiki/Image:PH_5_22217anneliese.PNG]] | |||
pH 5 Selected View | |||
[[Image:PH 5 view anneliese faustino22217.PNG]] | |||
pH 6 | |||
[[Image:PH 6 22217anneliese.PNG]] | |||
pH 6 Selected View | |||
[[Image:PH 6 view 22217annleiese.PNG]] | |||
pH 7 | |||
[[Image:PH 7 aanneliese22217.PNG]] | |||
pH 7 Selected View | |||
[[Image:PH 7 view anneliese faustino22217.PNG]] | |||
pH 8 | |||
[[Image:PH 8 22217annnnneneneliese.PNG]] | |||
pH 8 Selected View | |||
[[Image:PH 8 view 22217anneliese.PNG]] | |||
pH 9 | |||
[[Image:PH 9 22217anneliese.PNG]] | |||
pH 9 Selected View | |||
[[Image:PH 9 view anneliese22217.PNG]] | |||
pH 10 | |||
[[Image:PH 10 22217anneliesemarie.PNG]] | |||
pH 10 Selected View | |||
[[Image:PH 10 view 22217anneliesemariefaustino.PNG]] | |||
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BSA Templated CdS Quantum Dot synthesis and Myoglobin templated ZnS quantum dots dataCdS quantum dots were synthesized in a BSA template and fluorescence scans were run on the previously synthesized myoglobin templated ZnS quantum dots. Procedure
Myoglobin templated ZnS Quantum dotsScans taken of myoglobin templated ZnS quantum dots pH 4-10 NotesSince the myoglobin templated CdS quantum dots did not appear to have fully formed after stirring for one day, scans were only taken after stirring for 7 days. OverviewData Analysis: In DepthpH 5 [[1]]
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