IGEM:American University/2009/Notebook/CHEM 672/2017/02/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==''' | =='''Myoglobin Templated ZnS QDs'''== | ||
* | * '''Sample Preparation | ||
''' | |||
1. 400 µL of 5 mM Zn(CH<sub>3</sub>COO)<sub>2</sub> was mixed with 1600 µL of 5 mM NH<sub>4</sub>(CH<sub>3</sub>COO). | |||
2. The pH of the solutions was adjusted to the different values by using 10 mM CH<sub>3</sub>COOH for the pH 5 and 6, 100 mM CH<sub>3</sub>COOH for the pH 4, 10 mM NH<sub>4</sub>OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. | |||
3. 43.5 µL of 230 µM myoglobin was added to the solution and the total volume was adjusted to 4.6 mL. | |||
4. 1 hour incubation at 25°C. | |||
5. 400 µL of Na<sub>2</sub>S was added dropwise. | |||
6. The solution was stirred vigorously for 10 min at 25°C. | |||
7. Long-term sample storage at 4°C for 7 days. | |||
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[[Image:Data.Marjan's lab notebook.png]] | [[Image:Data.Marjan's lab notebook.png]] | ||
Latest revision as of 02:14, 27 September 2017
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Myoglobin Templated ZnS QDs
1. 400 µL of 5 mM Zn(CH3COO)2 was mixed with 1600 µL of 5 mM NH4(CH3COO). 2. The pH of the solutions was adjusted to the different values by using 10 mM CH3COOH for the pH 5 and 6, 100 mM CH3COOH for the pH 4, 10 mM NH4OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. 3. 43.5 µL of 230 µM myoglobin was added to the solution and the total volume was adjusted to 4.6 mL. 4. 1 hour incubation at 25°C. 5. 400 µL of Na2S was added dropwise. 6. The solution was stirred vigorously for 10 min at 25°C. 7. Long-term sample storage at 4°C for 7 days. .
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