IGEM:American University/2009/Notebook/CHEM 672/2017/02/15: Difference between revisions
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=='''Sample preparation'''== | =='''Sample preparation'''== | ||
* 1. 400 µL of 5 mM Zn(CH<sub>3</sub>COO)<sub>2</sub> was mixed with 1600 µL of 5 mM NH<sub>4</sub>(CH<sub>3</sub>COO). | * 1. 400 µL of 5 mM Zn(CH<sub>3</sub>COO)<sub>2</sub> was mixed with 1600 µL of 5 mM NH<sub>4</sub>(CH<sub>3</sub>COO). | ||
2. The pH of the solutions was adjusted to the different values by using 10 mM CH<sub>3</sub>COOH for the pH 5 and 6, 100 mM CH<sub>3</sub>COOH for the pH 4, 10 mM NH<sub>4</sub>OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. | 2. The pH of the solutions was adjusted to the different values by using 10 mM CH<sub>3</sub>COOH for the pH 5 and 6, 100 mM CH<sub>3</sub>COOH for the pH 4, 10 mM NH<sub>4</sub>OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. | ||
3. 43.5 µL of 230 µM myoglobin was added to the solution and the total volume was adjusted to 4.6 mL. | 3. 43.5 µL of 230 µM myoglobin was added to the solution and the total volume was adjusted to 4.6 mL. | ||
4. 1 hour incubation at 25°C. | 4. 1 hour incubation at 25°C. | ||
5. 400 µL of Na<sub>2</sub>S was added dropwise. | 5. 400 µL of Na<sub>2</sub>S was added dropwise. | ||
6. The solution was stirred vigorously for 10 min at 25°C. | 6. The solution was stirred vigorously for 10 min at 25°C. | ||
7. Long-term sample storage at 4°C for 7 days. | 7. Long-term sample storage at 4°C for 7 days. | ||
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Sample preparation
2. The pH of the solutions was adjusted to the different values by using 10 mM CH3COOH for the pH 5 and 6, 100 mM CH3COOH for the pH 4, 10 mM NH4OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. 3. 43.5 µL of 230 µM myoglobin was added to the solution and the total volume was adjusted to 4.6 mL. 4. 1 hour incubation at 25°C. 5. 400 µL of Na2S was added dropwise. 6. The solution was stirred vigorously for 10 min at 25°C. 7. Long-term sample storage at 4°C for 7 days. .
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