IGEM:American University/2009/Notebook/CHEM 672/2017/04/05: Difference between revisions
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(Autocreate 2017/04/05 Entry for IGEM:American_University/2009/Notebook/CHEM_672) |
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== | ==BSA Templated ZnS QDs== | ||
* | * Sample Preparation: | ||
1. 400 µL of 5 mM Zn(CH3COO)2 was mixed with 1600 µL of 5 mM NH4(CH3COO). | |||
2. The pH of the solutions was adjusted to the different values by using 10 mM CH3COOH for the pH 5 and 6, 100 mM CH3COOH for the pH 4, 10 mM NH4OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. | |||
3. 158.47 µL of 63.103 µM BSA was added to the solution and the total volume was adjusted to 4.6 mL. | |||
4. 1 hour incubation at 25°C. | |||
5. 400 µL of Na2S was added dropwise. | |||
6. The solution was stirred vigorously for 10 min at 25°C. | |||
7. Long-term sample storage at 4°C. | |||
[[Image:BSA prep.png]] | |||
Revision as of 21:39, 16 April 2017
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BSA Templated ZnS QDs
1. 400 µL of 5 mM Zn(CH3COO)2 was mixed with 1600 µL of 5 mM NH4(CH3COO). 2. The pH of the solutions was adjusted to the different values by using 10 mM CH3COOH for the pH 5 and 6, 100 mM CH3COOH for the pH 4, 10 mM NH4OH for the pH 7 and 8, and 100 mM NaOH for the pH 9 and 10. 3. 158.47 µL of 63.103 µM BSA was added to the solution and the total volume was adjusted to 4.6 mL. 4. 1 hour incubation at 25°C. 5. 400 µL of Na2S was added dropwise. 6. The solution was stirred vigorously for 10 min at 25°C. 7. Long-term sample storage at 4°C.
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