IGEM:American University/2009/Notebook/Chem 672/2017/02/22

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BSA Templated CdS Quantum Dot synthesis

CdS quantum dots were synthesized in a BSA template

Procedure

  1. A Cadmium acetate dihydrate solution was made to replace the previous stock solution of cadmium acetate anyhydrous because the cadmium acetate came out of solution and was not uniform.
    1. 0.666g of cadmium acetate dihydrous was dissolved in a 500mL volumetric flask. The stock solution is 5mM.
  2. Sample was made BSA: 0.1mM cadmium acetate, 3.125uM BSA, 10mM potassium thioacetate 5mL total
    1. 0.5M potassium thioacetate
      1. (10mM)(5.0mL)=(XmL)(0.5mM)
      2. XmL= 100uL potassium thioacetate
    2. BSA 63.1uM
      1. (3.125uM)(5.0mL)=(XmL)(63.1uM)
      2. X=0.247mL= 247uL BSA
    3. Cadmium Acetate 0.5M
      1. (0.1mM)(5.0mL)=(XmL)(50mM)
      2. X= 500uL
    4. Water
      1. Volume of water= 5000uL- 100uL-247uL-500uL= 4153uL

Overview

Scans taken of myoglobin templated ZnS quantum dots pH 4-10

Notes

Since the myoglobin templated CdS quantum dots did not appear to have fully formed after stirring for one day, scans were only taken after stirring for 7 days.



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