IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/01: Difference between revisions

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* Insert ladder as well as DNA plasmids in wells and let sit for around 45-50 minutes.  
* Insert ladder as well as DNA plasmids in wells and let sit for around 45-50 minutes.  
* This was to determine concentration of plasmids
* This was to determine concentration of plasmids
*Next, the sizes of the plasmids were tested to see how accurate they are compared to the literature value
**Entry by Vallari Somayaji
**Entry by Vallari Somayaji


Revision as of 13:22, 1 July 2014

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7/1/14 Gel Electrophoresis

  • Gel Electrophoresis of Plasmids
  • Made TAE buffer with 1 L of distilled water and 20mL of 50X stock of TAE. Also prepared the gel with agarose and water mixture and let solidify.
  • Made plasmids ready by using 3μL of DNA and 3μL of loading dye. 5 tubes of this were made as well as 1 tube of ladder 3μL.
  • Insert ladder as well as DNA plasmids in wells and let sit for around 45-50 minutes.
  • This was to determine concentration of plasmids
  • Next, the sizes of the plasmids were tested to see how accurate they are compared to the literature value
    • Entry by Vallari Somayaji


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