IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/01: Difference between revisions
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*2μL of DNA, 1μL of Buffer, 5μL of water, 2μL of, 1μL of E and 1μ of P restriction digests. | *2μL of DNA, 1μL of Buffer, 5μL of water, 2μL of, 1μL of E and 1μ of P restriction digests. | ||
* Heated in a 37°C water bath for around 40 minutes | * Heated in a 37°C water bath for around 40 minutes | ||
* Plasmids that have been cut were taken out and loading dye was input--2μL | |||
*This along with the ladder was filled into the wells and the machine was run for around 35 minutes. The voltage was increased from 80 V to 100 V | |||
* Pictures were taken of both gels and data recorded. | |||
**Entry by Vallari Somayaji | **Entry by Vallari Somayaji |
Revision as of 16:45, 1 July 2014
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7/1/14 Gel Electrophoresis
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