IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/01: Difference between revisions
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Line 30: | Line 30: | ||
||| DNA(plasmid) || 5.0 μL | ||| DNA(plasmid) || 5.0 μL | ||
|- | |- | ||
||| 10X buffer || 2 | ||| 10X buffer || 2.0 | ||
|- | |- | ||
||| dH<sub>2</sub>O || 13 | ||| dH<sub>2</sub>O || 13.0 | ||
|- | |- | ||
||| || 20 μL --> 37°C/ ~40 min. | ||| || 20.0 μL --> 37°C/ ~40 min. | ||
|} | |} | ||
Line 48: | Line 48: | ||
||| DNA(plasmid) || 5.0 μL | ||| DNA(plasmid) || 5.0 μL | ||
|- | |- | ||
||| 10X buffer || 2 | ||| 10X buffer || 2.0 | ||
|- | |- | ||
||| EcoRI || 1.0 | ||| EcoRI || 1.0 | ||
Line 54: | Line 54: | ||
||| PstI || 1.0 | ||| PstI || 1.0 | ||
|- | |- | ||
||| dH<sub>2</sub>O || 11 | ||| dH<sub>2</sub>O || 11.0 | ||
|- | |- | ||
||| || 20 μL --> 37°C/ ~40 min. | ||| || 20.0 μL --> 37°C/ ~40 min. | ||
|} | |} | ||
Revision as of 10:41, 15 July 2014
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7/1/14 Gel Electrophoresis
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