IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Ligation Reactions==
==Ligation Reactions==
*Began design, but did not begin reaction
* Independently digest the TesA (sample 1), RBS (sample 7) to combine with provided backbone (24KS EPKS)which has been cut at the E and P sites.
* Independently digest the TesA (sample 1), RBS (sample 7) to combine with provided backbone (24KS EPKS)which has been cut at the E and P sites.


Revision as of 19:58, 2 July 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Ligation Reactions

  • Began design, but did not begin reaction
  • Independently digest the TesA (sample 1), RBS (sample 7) to combine with provided backbone (24KS EPKS)which has been cut at the E and P sites.

TesA

  • Digest 5 μL DNA with 1 μL buffer, 1μL Xba1, 1 μL Pst1, and 2μL of water.

RBS

  • Digest 5 μL DNA with 1 μL buffer, 1μL EcoR1, 1 μL Spe1, and 2μL of water.

Ligation

Transformation

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Arizona_State/2014/Notebook/asu_igem_2014/2014/07/02&oldid=799840"