IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/07: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Gel Extraction== | ||
* | * Ran gel extraction on gel from 7/4 | ||
Procedure: | |||
* Cut selected fragments out from the gel | |||
* Dissolve in ADB buffer. For the buffer, 3x the volume (measured by weight) of the gel was added. Place on a heat block to dissolve | |||
* | |||
Issues with the gel indicate that one or more enzymes may not be functioning correctly. | |||
Enzyme Testing: | |||
* Tested enzymes with MCherry | |||
* Ran digests with single cuts for X P S and E on MCherry as well as MCherry with cuts on X and P, E and S, and X and S, and E and S. | |||
*Let run overnight | |||
Latest revision as of 00:05, 27 September 2017
Project name | Main project page Previous entry Next entry |
Gel Extraction
Procedure:
Issues with the gel indicate that one or more enzymes may not be functioning correctly. Enzyme Testing:
|