IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/08: Difference between revisions
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== | ==Gel Extraction and New Digests== | ||
* The Mcherry plate posed a few problems with enzymes, but the cuts had been made properly. It showed that the P site does not cut properly and usually results in partial digestions. | * The Mcherry plate posed a few problems with enzymes, but the cuts had been made properly. It showed that the P site does not cut properly and usually results in partial digestions. | ||
* Made a new game plan for getting a Kanamyacin cell with three plasmids, RBS, TES, and MCherry. | * Made a new game plan for getting a Kanamyacin cell with three plasmids, RBS, TES, and MCherry. |
Revision as of 14:04, 8 July 2014
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Gel Extraction and New Digests
Ligate: TesA part into RBS backbone part E|X| RBS | S| X| TESA | P| P| Transform: Transform the plasmids into cells (mix plasmids with competent cells) Plate Cells
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