IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/15: Difference between revisions
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==Entry title== | ==Entry title== | ||
* | * Checked DNA concentrations | ||
:TesA= 11.99 ng/μL ; RBS=32.99 ng/μL | |||
*When divided by the number of base pairs: [TesA]=.018 strands/μL and [RBS]=.016 strands/μL | |||
* Ligation | * Ligation | ||
{| {{table}} border="1" cellspacing="3" align=center <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(plasmid) || 1.0 μL RBS, 2.0 μL TesA | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 5.0 | |||
|- | |||
|| Ligaze || 1.0 | |||
|- | |||
|| || 10.0 μL --> 25°C/ ~1 Hr. | |||
|} | |||
* Transformation | * Transformation | ||
Revision as of 14:31, 15 July 2014
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