IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/15: Difference between revisions
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*After ligation, the mixture was heated to 65°C for 10 min. to denature the ligaze. | *After ligation, the mixture was heated to 65°C for 10 min. to denature the ligaze. | ||
*Transformation was then performed. | *Transformation was then performed. | ||
:Three plates were grown overnight: RBS backbone (vector), TesA+RBS, and mCherryboth as a positive control and to replenish our stocks. | |||
* Transformation | * Transformation | ||
:Unfortunately, no growth was seen on any of the plates, including the positive control of mCherry. | |||
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Revision as of 07:46, 16 July 2014
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TesA/RBS Ligation Attempt 2
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