IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#Competent cells: | #Competent cells: | ||
#*Mixed 50 μL of competent cells, treated with the mCherry plasmid, with 500 μL of SOC and .5 μL of chloramphenicol, and incubated @ 32°C | #*Mixed 50 μL of competent cells, treated with the mCherry plasmid, with 500 μL of SOC and .5 μL of chloramphenicol, and incubated @ 32°C | ||
::After about 2 hours, no growth was seen. | |||
#*Poured remaining mixture of mCherry competent cells onto a new chlor. plate and incubated @ 32°C | #*Poured remaining mixture of mCherry competent cells onto a new chlor. plate and incubated @ 32°C | ||
#Ligation | #Ligation | ||
#*Started a new ligation using the exact same measurements as yesterday (7/15) with the same positive control (mCherry) and negative control (RBS backbone). | #*Started a new ligation using the exact same measurements as yesterday (7/15) with the same positive control (mCherry) and negative control (RBS backbone). | ||
#*Changes: ligaze was not denatured in PCR | |||
#*Theoretically, today's competent cells are not compromised in any way and will grow properly | #*Theoretically, today's competent cells are not compromised in any way and will grow properly | ||
Latest revision as of 00:07, 27 September 2017
Project name | Main project page Previous entry Next entry |
Ligation attempt # 3
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