IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*Yesterdays streaked plates from the third ligation showed no growth, except for mCherry, which had maybe 15 very small colonies. | *Yesterdays streaked plates from the third ligation showed no growth, except for mCherry, which had maybe 15 very small colonies. | ||
*The mCherry that was poured out onto a new plate yesterday had a full lawn. | *The mCherry that was poured out onto a new plate yesterday had a full lawn. | ||
*Also, the mCherry test of the competent cells grew very well. the 1.5 ml tube had been left in the incubator over night. | |||
**Considering this information, the remaining samples of the TesA/RBS ligation and the RBS backbone were poured onto new plates in the hopes that they too will show more growth. | **Considering this information, the remaining samples of the TesA/RBS ligation and the RBS backbone were poured onto new plates in the hopes that they too will show more growth. | ||
**Plates were left upstairs in the 32°C incubator, as the incubator downstairs doesn't hold a constant temp, and its airflow drys out the plates prematurely. | **Plates were left upstairs in the 32°C incubator, as the incubator downstairs doesn't hold a constant temp, and its airflow drys out the plates prematurely. |
Latest revision as of 00:07, 27 September 2017
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Ligation #3 Continued
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