IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/21: Difference between revisions
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(Autocreate 2014/07/21 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
(fix raw html notebook nav) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==7/21== | ||
* | * Ligations started for TES with RBS and control. Following are the tables. | ||
{| {{table}} border="1" align=center <!-- Ligation table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(plasmid) || 1.0 μL RBS, 2.0 μL TesA | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 5.0 | |||
|- | |||
|| Ligaze || 1.0 | |||
|- | |||
|| || 10.0 μL --> 16°C/ ~overnight | |||
|} | |||
{| {{table}} border="1" align=center <!-- Ligation table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(plasmid) || 1.0 μL RBS, 0 μL TesA | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 7.0 | |||
|- | |||
|| Ligaze || 1.0 | |||
|- | |||
|| || 10.0 μL --> 16°C/ overnight | |||
|} | |||
* Transformations of Tet (Tube 4) on competency cells. | |||
* 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} |
Latest revision as of 00:07, 27 September 2017
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