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- Ligations started for TES with RBS and control. Following are the tables.
Reagent (μL)
|
Volume (μL)
|
DNA(plasmid) |
1.0 μL RBS, 2.0 μL TesA
|
10X buffer |
1.0
|
dH2O |
5.0
|
Ligaze |
1.0
|
|
10.0 μL --> 16°C/ ~overnight
|
Reagent (μL)
|
Volume (μL)
|
DNA(plasmid) |
1.0 μL RBS, 0 μL TesA
|
10X buffer |
1.0
|
dH2O |
7.0
|
Ligaze |
1.0
|
|
10.0 μL --> 16°C/ overnight
|
- Transformations of Tet (Tube 4) on competency cells.
- 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates.
|