IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/22: Difference between revisions

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* Started seeds of ethanol
* Started seeds of ethanol
* Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
* Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
Plate 1: 1 μl of Tet with DNA (positive control)
* Plate 1: 1 μl of Tet with DNA (positive control)
Plate 2: 1μl of ligation mixture
* Plate 2: 1μl of ligation mixture
Plate 3: 3 μl of ligation mixture
* Plate 3: 3 μl of ligation mixture
Plate 4: 6 μl of ligation mixture  
* Plate 4: 6 μl of ligation mixture  
Plate 5: 1 μl RBS Backbone (negative control)
* Plate 5: 1 μl RBS Backbone (negative control)
Plate 6:  3 μl RBS backbone
* Plate 6:  3 μl RBS backbone
Plate 7: 6 μl RBS Backbone  
* Plate 7: 6 μl RBS Backbone  





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  • Started seeds of ethanol
  • Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
  • Plate 1: 1 μl of Tet with DNA (positive control)
  • Plate 2: 1μl of ligation mixture
  • Plate 3: 3 μl of ligation mixture
  • Plate 4: 6 μl of ligation mixture
  • Plate 5: 1 μl RBS Backbone (negative control)
  • Plate 6: 3 μl RBS backbone
  • Plate 7: 6 μl RBS Backbone