IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/22

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  • Started seeds of ethanol
  • Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
  • Plate 1: 1 μl of Tet with DNA (positive control)
  • Plate 2: 1μl of ligation mixture
  • Plate 3: 3 μl of ligation mixture
  • Plate 4: 6 μl of ligation mixture
  • Plate 5: 1 μl RBS Backbone (negative control)
  • Plate 6: 3 μl RBS backbone
  • Plate 7: 6 μl RBS Backbone


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