IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30

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(Autocreate 2014/07/30 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
Current revision (17:12, 1 August 2014) (view source)
(Wednesday 7/30)
 
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==Entry title==
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==Wednesday 7/30==
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* Insert content here...
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* Mini Prep:
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: Second attempt
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: Isolated stocks of TesA+RBS and LacI plasmids
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* Gel Extraction:
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: TesA+RBS cut at X and P sites
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: LacI cut at S and P sites
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: 260/280 ratio for LacI is shady, ~1.002
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* Ligation:
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: TesA+RBS insert with LacI+backbone
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{| {{table}} border="1" align=center <!-- Digestion table -->
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent
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| bgcolor=#cfcfcf | 1 (μL)
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| bgcolor=#cfcfcf | 2 (μL)
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| bgcolor=#cfcfcf | Control (μL)
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|-
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|| TesA+RBS || 2.0 || 2.0 || 0.0
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|-
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|| LacI || 3.0 || 4.0 || 3.0
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|-
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|| Ligation Buffer || 1.0 || 1.0 || 1.0
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|-
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|| Ligase || 1.0 || 1.0 || 1.0
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|-
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|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0
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|-
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Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone
Reagent 1 (μL) 2 (μL) Control (μL)
TesA+RBS 2.0 2.0 0.0
LacI 3.0 4.0 3.0
Ligation Buffer 1.0 1.0 1.0
Ligase 1.0 1.0 1.0
H2O 3.0 2.0 5.0
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