IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30: Difference between revisions
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* Ligation: | * Ligation: | ||
: TesA+RBS insert with LacI+backbone | : TesA+RBS insert with LacI+backbone | ||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | 1 (μL) | |||
| bgcolor=#cfcfcf | 2 (μL) | |||
| bgcolor=#cfcfcf | Control (μL) | |||
|- | |||
|| TesA+RBS || 2.0 || 2.0 || 0.0 | |||
|- | |||
|| LacI || 3.0 || 4.0 || 3.0 | |||
|- | |||
|| Ligation Buffer || 1.0 || 1.0 || 1.0 | |||
|- | |||
|| Ligase || 1.0 || 1.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0 | |||
|- | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 14:12, 1 August 2014
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Wednesday 7/30
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