IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 18: Line 18:
* Ligation:
* Ligation:
: TesA+RBS insert with LacI+backbone
: TesA+RBS insert with LacI+backbone
:
{| {{table}} border="1" align=center <!-- Digestion table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | 1 (μL)
| bgcolor=#cfcfcf | 2 (μL)
| bgcolor=#cfcfcf | Control (μL)
|-
|| TesA+RBS || 2.0 || 2.0 || 0.0
|-
|| LacI || 3.0 || 4.0 || 3.0
|-
|| Ligation Buffer || 1.0 || 1.0 || 1.0
|-
|| Ligase || 1.0 || 1.0 || 1.0
|-
|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0
|-


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 14:12, 1 August 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone
Reagent 1 (μL) 2 (μL) Control (μL)
TesA+RBS 2.0 2.0 0.0
LacI 3.0 4.0 3.0
Ligation Buffer 1.0 1.0 1.0
Ligase 1.0 1.0 1.0
H2O 3.0 2.0 5.0
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Arizona_State/2014/Notebook/asu_igem_2014/2014/07/30&oldid=804764"