IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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|| 37°C for 1 hour | || 37°C for 1 hour | ||
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*Both samples used in the Clean and Concentrate kit | *Both samples used in the Clean and Concentrate kit | ||
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|| 37°C for 1 hour then 65°C for 5 min | || 37°C for 1 hour then 65°C for 5 min | ||
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*The RBS-TesA and LacI DNA samples were quantified: | *The RBS-TesA and LacI DNA samples were quantified: | ||
RBS-TesA: 39.157 ng/μL | *RBS-TesA: 39.157 ng/μL | ||
LacI: 62.579 ng/μL | *LacI: 62.579 ng/μL | ||
*Overnight ligation reactions were conducted for the test ligation and the negative control. | *Overnight ligation reactions were conducted for the test ligation and the negative control. | ||
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|| 16°C overnight | || 16°C overnight | ||
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<u>Ethanol:</u> | |||
*Started seeds of 3 seperate colonies of ethanol plasmid | |||
**50 ml LB w/ Chloramphenicol + picked colony | |||
**Cells didn't grow fast enough to add IPTG to start protein growth, so cells were left overnight to grow more | |||
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Latest revision as of 00:09, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Preparing another RBS-TesA and LacI Ligation
Ethanol:
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