IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/04

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(Autocreate 2014/08/04 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
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==Entry title==
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==Preparing another RBS-TesA and LacI Ligation==
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* Insert content here...
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* Digested more of RBS-TesA with X and P(insert). Digested more of LacI with S and P (backbone).
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*Treat the LacI with Antarctic Phsophotase.
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*The RBS-TesA and LacI DNA samples were quantified:
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RBS-TesA: 39.157 ng/μL
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LacI: 62.579 ng/μL
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*Overnight ligation reactions were conducted for the test ligation and the negative control.
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Revision as of 13:55, 5 August 2014

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Preparing another RBS-TesA and LacI Ligation

  • Digested more of RBS-TesA with X and P(insert). Digested more of LacI with S and P (backbone).
  • Treat the LacI with Antarctic Phsophotase.
  • The RBS-TesA and LacI DNA samples were quantified:

RBS-TesA: 39.157 ng/μL LacI: 62.579 ng/μL

  • Overnight ligation reactions were conducted for the test ligation and the negative control.
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