IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05: Difference between revisions

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==Entry title==
==7/5 ==
* Insert content here...
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
<u>Ethanol:</u>
*Started shake flasks of seeds started yesterday.
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
**Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
**Let grow overnight




Latest revision as of 00:09, 27 September 2017

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7/5

  • Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.

Ethanol:

  • Started shake flasks of seeds started yesterday.
    • Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
    • Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
    • Let grow overnight


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