IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05

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(7/5)
 
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==7/5 ==
==7/5 ==
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
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* Started seeds of ethanol
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<u>Ethanol:</u>
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*Started shake flasks of seeds started yesterday.
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**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
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**Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
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**Let grow overnight

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7/5

  • Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.

Ethanol:

  • Started shake flasks of seeds started yesterday.
    • Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
    • Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
    • Let grow overnight


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