IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→7/5) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 8: | Line 8: | ||
==7/5 == | ==7/5 == | ||
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control. | * Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control. | ||
* Started seeds | <u>Ethanol:</u> | ||
*Started shake flasks of seeds started yesterday. | |||
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol | |||
**Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM) | |||
**Let grow overnight | |||
Revision as of 20:33, 6 August 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
7/5
Ethanol:
| |
