IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/06: Difference between revisions
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*Obtained acc plasmid (chloro resistance). Started plate and liquid colonies. | *Obtained acc plasmid (chloro resistance). Started plate and liquid colonies. | ||
<u>Ethanol:</u> | |||
*At 8:30 AM, O.D. of cells was approx. 1.2 | |||
**Resuspended in 30 ml PBS, adjusted O.D. to match at 1.0, resuspended in PBS again | |||
**Began resting cell assay (9:15 AM): | |||
:::-Flask 1: 1 g/L pyruvate (.03 g) | |||
:::-Flask 2: 3 g/L pyruvate (.09 g)+ 1 g/L glucose (.03 g) | |||
:::-Flask 3: 3 g/L pyruvate (.09 g)(no glucose) | |||
**Flasks were kept in 32°C shaker/incubator with 1 ml samples taken for Hrs. 1,2,3,6, and 8. (24 Hr. sample will be taken tomorrow) | |||
*Made standards of ethanol and pyruvate to be run on the HPLC: | |||
**Standards were first made of the max possible amount of each substance present in the system. (in 30 ml) These concentrations were then used as a stock to be diluted for subsequent concentrations | |||
{| {{table}} border="1" class="wikitable" style="text-align:center; width:400px; height:200px;"<!-- Ethanol Standard Table --> | |||
|+Ethanol Standard | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Desired Concentration (mol/L) | |||
| bgcolor=#cfcfcf | Water Added (ml) | |||
| bgcolor=#cfcfcf | Pure Ethanol Added (ml) | |||
|- | |||
| bgcolor=#cfcfcf | 1.13 | |||
| bgcolor=#cfcfcf | 28.021 | |||
| bgcolor=#cfcfcf | 1.979 | |||
|- | |||
|| .757|| .33 || .620 | |||
|- | |||
|| .38 || .664 || .336 | |||
|- | |||
|| .3|| .735 || .265 | |||
|- | |||
|| .2 || .823 || .177 | |||
|- | |||
|| .1 || .912 || .088 | |||
|- | |||
|} | |||
{| {{table}} border="1" class="wikitable" style="text-align:center; width:400px; height:200px;"<!-- Pyruvate Standard Table --> | |||
|+Pyruvate Standard | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Desired Concentration (g/L) | |||
| bgcolor=#cfcfcf | Water Added (ml) | |||
| bgcolor=#cfcfcf | Pyruvate Added (ml) | |||
|- | |||
| bgcolor=#cfcfcf | 3.0 | |||
| bgcolor=#cfcfcf | 30 ml | |||
| bgcolor=#cfcfcf | .09 g | |||
|- | |||
|| 2.0 || .333 || .667 | |||
|- | |||
|| 1.0 || .666 || .333 | |||
|- | |||
|| .7 || .767 || .233 | |||
|- | |||
|| .5 || .833 || .167 | |||
|- | |||
|| .3 || .900 || .100 | |||
|- | |||
|} | |||
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Revision as of 20:51, 6 August 2014
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Entry title
Ethanol:
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