IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/06: Difference between revisions
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*Obtained acc plasmid (chloro resistance). Started plate and liquid colonies. | *Obtained acc plasmid (chloro resistance). Started plate and liquid colonies. | ||
ETHANOL: | |||
*At 8:30 AM, O.D. of cells was approx. 1.2 | |||
**Resuspended in 30 ml PBS, adjusted O.D. to match at 1.0, resuspended in PBS again | |||
**Began resting cell assay (9:15 AM): | |||
:::-Flask 1: 1 g/L pyruvate (.03 g) | |||
:::-Flask 2: 3 g/L pyruvate (.09 g)+ 1 g/L glucose (.03 g) | |||
:::-Flask 3: 3 g/L pyruvate (.09 g)(no glucose) | |||
**Flasks were kept in 32°C shaker/incubator with 1 ml samples taken for Hrs. 1,2,3,6, and 8. (24 Hr. sample will be taken tomorrow) | |||
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Revision as of 20:03, 6 August 2014
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ETHANOL:
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