IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/11: Difference between revisions
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==LacI + RBA-TesA ligation== | ==LacI + RBA-TesA ligation== | ||
* Antarctic phosphotase treatment of LacI sample | * Antarctic phosphotase treatment of LacI sample | ||
: Antarctic Phsophotase Treatment | |||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | | |||
|- | |||
|| LacI DNA || 10.0 | |||
|- | |||
|| Antarctic Phosphotase|| 1.0 | |||
|- | |||
|| Phosphotase Buffer || 2.0 | |||
|- | |||
|| H<sub>2</sub>O || 7.0 | |||
|- | |||
|| 37°C for 1 hour then 65°C for 5 min | |||
|- | |||
*Gel Extraction of RBS-TesA part: Sample was split evenly between 2 wells. Each well was collected separately and run through the standard procedure of the extraction kit (Zippy). | *Gel Extraction of RBS-TesA part: Sample was split evenly between 2 wells. Each well was collected separately and run through the standard procedure of the extraction kit (Zippy). |
Revision as of 15:15, 11 August 2014
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LacI + RBA-TesA ligation
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