IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/11: Difference between revisions
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*Ligation reaction | *Ligation reaction | ||
: TesA+RBS insert with LacI+backbone | |||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | 1 (μL) | |||
| bgcolor=#cfcfcf | Control (μL) | |||
|- | |||
|| TesA+RBS || 2.0 || 0.0 | |||
|- | |||
|| LacI || 2.0 || 2.0 | |||
|- | |||
|| Ligation Buffer || 1.0 || 1.0 | |||
|- | |||
|| Ligase || 1.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 4.0 || 6.0 | |||
|- | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} |
Revision as of 15:17, 11 August 2014
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LacI + RBA-TesA ligation
|