IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/12: Difference between revisions
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##+ Control (ACC Plasmid) | ##+ Control (ACC Plasmid) | ||
PCR Amplification of ACC | PCR Amplification of ACC parts <br style="clear:both;"/><br> | ||
First, the dry primers were made into 100 μM master stocks. 1 μL was added per .1 nm. For example, a 31.2 nm sample had 312 μL added. This master stock was then diluted into working stocks of 10 μM. This was done by adding 1 μL of master mix into 9 μL H2O. This was done for all eight primers , four forward and four reverse. | |||
The ACC plasmid was then diluted from 54.2 μg/μL to about 1 μg/μL through a 1/50 dilution. | |||
PCR mix for all four reactions (accA, accB, accC, and accD) | |||
<u>Ethanol</u> | <u>Ethanol</u> | ||
Revision as of 10:57, 15 August 2014
 Project name
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PCR Amplification of ACC parts
Ethanol Got plasmid PACYC Duet-pdc-adhb in strain DH10b and strain BL21 from Nielsen's lab to use for ethanol production
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