IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/15: Difference between revisions

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==8/15==
==8/15==
Started flask of DH10b cells for competency.
Started flask of DH10b cells for competency.
*Made new comp. cells following Nielsen Lab protocol for competent cells
<br>
<u>Ethanol</u><br>
<u>Ethanol</u><br>
Transformed PACYC plasmid into BL21 cell line.
Transformed PACYC plasmid into BL21 cell line.
:-no growth was seen on PACYC plasmid plate. need to run a control of competent cells.





Revision as of 11:54, 18 August 2014

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8/15

Started flask of DH10b cells for competency.

  • Made new comp. cells following Nielsen Lab protocol for competent cells


Ethanol
Transformed PACYC plasmid into BL21 cell line.

-no growth was seen on PACYC plasmid plate. need to run a control of competent cells.