IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/18: Difference between revisions

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(Autocreate 2014/08/18 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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==Entry title==
==8/18==
* Insert content here...
TesA+RBS:
*Transformed ligation that was in freezer.
ACC Plasmid:
*Ran a gel of amplified plasmid parts to ensure that the PCR worked
*Used clean and concentrator kit to purify
 
Ethanol:
*Re-transformed PACYC plasmid into BL21 cell line.
*Started positive control of PACYC to test competency.  




Revision as of 12:06, 18 August 2014

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8/18

TesA+RBS:

  • Transformed ligation that was in freezer.

ACC Plasmid:

  • Ran a gel of amplified plasmid parts to ensure that the PCR worked
  • Used clean and concentrator kit to purify

Ethanol:

  • Re-transformed PACYC plasmid into BL21 cell line.
  • Started positive control of PACYC to test competency.


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