IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/19: Difference between revisions

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*PACYC plate had no growth. Started a positive control to ensure the competency of the BL21 cells.
*PACYC plate had no growth. Started a positive control to ensure the competency of the BL21 cells.
**Positive control DNA was TesA on chlor vector
**Positive control DNA was TesA on chlor vector
 
***3 μL of TesA in 50 μL competent cells


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Revision as of 09:05, 29 August 2014

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  • All amp. plates from yesterday had growth suggesting that vector DNA was all that was taken.
  • PACYC plate had no growth. Started a positive control to ensure the competency of the BL21 cells.
    • Positive control DNA was TesA on chlor vector
      • 3 μL of TesA in 50 μL competent cells
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