IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|

Latest revision as of 00:13, 27 September 2017

Project name Main project page
Previous entry      Next entry

8/25

  • Growth on Amp plates of ligation from 8/19 suggested that competent cells had contamination: did a test of cells to ensure no contamination
    • streaked Bl21 and Dh10b competent cells onto Amp plates: no growth on either.

Transformed K1122676 plasmid (ethanol plasmid from registry) into Bl21 cell line to run another assay. Transformed PACYC Duet vector into Bl21 cell line as well.

  • Both transformetions were done with 3 μL of Plasmid, and 50 μL of cells. recovered in 400 μL SOC.
  • three assays will be run: empty PACYC vector, PACYC Duet Pdc-Adhb, and K1122676.

Growth on transformation of PACYC Duet Pdc-Adhb had one colony.

  • started a seed to miniprep and run on a gel to ensure that we have the right part.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Arizona_State/2014/Notebook/asu_igem_2014/2014/08/25&oldid=1015169"