IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/02: Difference between revisions

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<u>Ethanol</u><br>
Not enough time to run Resting cell assay today. Will try again next week.


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Revision as of 18:21, 10 September 2014

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accD Gel Prep Work

  • Product from Final accD PCR was cleaned and concentrated using a Zymo kit (no change to procedure, eluted in 25)
  • Digest SOE accD with EcoRI and Pst1 to check if E cut site was removed and to prepare for ligation with pSB1C3 digested at E and P
Ethanol
Not enough time to run Resting cell assay today. Will try again next week.
Reagent RBS-TesA (μL)
accD product 25.0
10X fast Digest Buffer 3.0
PstI 1.0
EcoRI 1.0
37°C for 10 hours
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